In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes.
Identifieur interne : 000255 ( Main/Exploration ); précédent : 000254; suivant : 000256In vitro steroidogenic effects of mixtures of persistent organic pollutants (POPs) extracted from burbot (Lota lota) caught in two Norwegian lakes.
Auteurs : Karin E. Zimmer [Norvège] ; Mauricio Monta O ; Ingrid Olsaker ; Ellen Dahl ; Vidar Berg ; Camilla Karlsson ; Albertinka J. Murk ; Janneche U. Skaare ; Erik Ropstad ; Steven VerhaegenSource :
- The Science of the total environment [ 1879-1026 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux (MeSH), DDT (métabolisme), Eau douce (composition chimique), Expression des gènes (effets des médicaments et des substances chimiques), Gadiformes (métabolisme), Gadiformes (physiologie), Gadiformes (sang), Hormones sexuelles stéroïdiennes (sang), Hydrocarbures bromés (métabolisme), Ignifuges (métabolisme), Lignée cellulaire (MeSH), Norvège (MeSH), Perturbateurs endocriniens (métabolisme), Perturbateurs endocriniens (toxicité), Polluants chimiques de l'eau (métabolisme), Polluants chimiques de l'eau (toxicité), Surveillance de l'environnement (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Éthers de polyhalogénophényle (métabolisme).
- MESH :
- composition chimique : Eau douce.
- effets des médicaments et des substances chimiques : Expression des gènes, Survie cellulaire.
- métabolisme : DDT, Gadiformes, Hydrocarbures bromés, Ignifuges, Perturbateurs endocriniens, Polluants chimiques de l'eau, Éthers de polyhalogénophényle.
- physiologie : Gadiformes.
- sang : Gadiformes, Hormones sexuelles stéroïdiennes.
- toxicité : Perturbateurs endocriniens, Polluants chimiques de l'eau.
- Animaux, Lignée cellulaire, Norvège, Surveillance de l'environnement.
- Wicri :
- geographic : Norvège.
English descriptors
- KwdEn :
- Animals (MeSH), Cell Line (MeSH), Cell Survival (drug effects), DDT (metabolism), Endocrine Disruptors (metabolism), Endocrine Disruptors (toxicity), Environmental Monitoring (MeSH), Flame Retardants (metabolism), Fresh Water (chemistry), Gadiformes (blood), Gadiformes (metabolism), Gadiformes (physiology), Gene Expression (drug effects), Gonadal Steroid Hormones (blood), Halogenated Diphenyl Ethers (metabolism), Hydrocarbons, Brominated (metabolism), Norway (MeSH), Water Pollutants, Chemical (metabolism), Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , blood : Gonadal Steroid Hormones.
- chemical , metabolism : DDT, Endocrine Disruptors, Flame Retardants, Halogenated Diphenyl Ethers, Hydrocarbons, Brominated, Water Pollutants, Chemical.
- chemical , toxicity : Water Pollutants, Chemical.
- geographic : Norway.
- blood : Gadiformes.
- chemistry : Fresh Water.
- drug effects : Cell Survival, Gene Expression.
- metabolism : Gadiformes.
- physiology : Gadiformes.
- chemical , toxicity : Endocrine Disruptors.
- Animals, Cell Line, Environmental Monitoring.
Abstract
This study investigated the effects of two mixtures of persistent organic pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes (Mjøsa and Losna) with different contaminant profiles. Steroid hormone levels in the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis were investigated. The crude Lake Mjøsa extract had to be diluted ten times more than the Lake Losna extract in order to prevent cytotoxicity. The ten times diluted Lake Mjøsa mixture had higher levels of DDT and derivates (∑DDTs, 1.7 times) and brominated flame retardants (∑BDEs and HBCD, 15-25 times) than the Lake Losna mixture, which, on the other hand, had higher concentrations of ∑PCBs (1.5 times higher) and also of HCB, ∑HCH isomers and ∑chlordane isomers (5-20 times higher). In the cell culture media, only cortisol levels were increased at the highest exposure concentration to the Lake Mjøsa mixture, while both cortisol and estradiol levels were increased following exposure to the two highest Lake Losna mixture exposure concentrations. Testosterone levels decreased only at the highest exposure concentration of the Lake Losna mixture. Multivariate models suggested that ∑PCBs, and to a lesser extent ∑DDTs, were responsible for the cortisol responses, while estradiol and testosterone alterations were best explained by HCB and ∑PCBs, respectively. Exposure to the mixtures generally increased mRNA levels, with smaller effects exerted by the Lake Mjøsa mixture than the Lake Losna mixture. It was concluded that both mixtures affected steroidogenesis in the H295R cells. Small differences in mixture composition, rather than the high content of brominated flame retardants in the Lake Mjøsa mixture, were suggested to be the most probable reason for the apparent differences in potencies of the two mixtures.
DOI: 10.1016/j.scitotenv.2011.01.055
PubMed: 21420147
Affiliations:
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xml:lang="en">This study investigated the effects of two mixtures of persistent organic pollutants (POPs) on steroidogenesis in the H295R cell line. The two mixtures were obtained from the livers of burbot (Lota lota) caught in two Norwegian lakes (Mjøsa and Losna) with different contaminant profiles. Steroid hormone levels in the cell culture medium and mRNA levels of 16 genes involved in steroidogenesis were investigated. The crude Lake Mjøsa extract had to be diluted ten times more than the Lake Losna extract in order to prevent cytotoxicity. The ten times diluted Lake Mjøsa mixture had higher levels of DDT and derivates (∑DDTs, 1.7 times) and brominated flame retardants (∑BDEs and HBCD, 15-25 times) than the Lake Losna mixture, which, on the other hand, had higher concentrations of ∑PCBs (1.5 times higher) and also of HCB, ∑HCH isomers and ∑chlordane isomers (5-20 times higher). In the cell culture media, only cortisol levels were increased at the highest exposure concentration to the Lake Mjøsa mixture, while both cortisol and estradiol levels were increased following exposure to the two highest Lake Losna mixture exposure concentrations. Testosterone levels decreased only at the highest exposure concentration of the Lake Losna mixture. Multivariate models suggested that ∑PCBs, and to a lesser extent ∑DDTs, were responsible for the cortisol responses, while estradiol and testosterone alterations were best explained by HCB and ∑PCBs, respectively. Exposure to the mixtures generally increased mRNA levels, with smaller effects exerted by the Lake Mjøsa mixture than the Lake Losna mixture. It was concluded that both mixtures affected steroidogenesis in the H295R cells. Small differences in mixture composition, rather than the high content of brominated flame retardants in the Lake Mjøsa mixture, were suggested to be the most probable reason for the apparent differences in potencies of the two mixtures.</div></front></TEI><affiliations><list><country><li>Norvège</li></country><region><li>Østlandet</li></region><settlement><li>Oslo</li></settlement></list><tree><noCountry><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name><name sortKey="Dahl, Ellen" sort="Dahl, Ellen" uniqKey="Dahl E" first="Ellen" last="Dahl">Ellen Dahl</name><name sortKey="Karlsson, Camilla" sort="Karlsson, Camilla" uniqKey="Karlsson C" first="Camilla" last="Karlsson">Camilla Karlsson</name><name sortKey="Monta O, Mauricio" sort="Monta O, Mauricio" uniqKey="Monta O M" first="Mauricio" last="Monta O">Mauricio Monta O</name><name sortKey="Murk, Albertinka J" sort="Murk, Albertinka J" uniqKey="Murk A" first="Albertinka J" last="Murk">Albertinka J. Murk</name><name sortKey="Olsaker, Ingrid" sort="Olsaker, Ingrid" uniqKey="Olsaker I" first="Ingrid" last="Olsaker">Ingrid Olsaker</name><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U" last="Skaare">Janneche U. Skaare</name><name sortKey="Verhaegen, Steven" sort="Verhaegen, Steven" uniqKey="Verhaegen S" first="Steven" last="Verhaegen">Steven Verhaegen</name></noCountry><country name="Norvège"><region name="Østlandet"><name sortKey="Zimmer, Karin E" sort="Zimmer, Karin E" uniqKey="Zimmer K" first="Karin E" last="Zimmer">Karin E. Zimmer</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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